Noncoded Amino Acids in de Novo Metalloprotein Design: Controlling Coordination Number and Catalysis
نویسندگان
چکیده
منابع مشابه
De novo design of a single-chain diphenylporphyrin metalloprotein.
We describe the computational design of a single-chain four-helix bundle that noncovalently self-assembles with fully synthetic non-natural porphyrin cofactors. With this strategy, both the electronic structure of the cofactor as well as its protein environment may be varied to explore and modulate the functional and photophysical properties of the assembly. Solution characterization (NMR, UV-v...
متن کاملIdentification of amino acids involved in protein structural uniqueness: implication for de novo protein design.
Structural uniqueness is characteristic of native proteins and is essential to express their biological functions. The major factors that bring about the uniqueness are specific interactions between hydrophobic residues and their unique packing in the protein core. To find the origin of the uniqueness in their amino acid sequences, we analyzed the distribution of the side chain rotational isome...
متن کامل158 Statistical fluctuations, dynamics, and de novo enzyme catalysis.
My talk will describe our recent theoretical developments in methods and models in quantifying protein statistical and dynamical motions and their influence on catalytic performance of de novo enzymes and the possibility of incorporating fluctuations and dynamics into the early stages of enzyme design.
متن کاملControlling complexity and water penetration in functional de novo protein design.
Natural proteins are complex, and the engineering elements that support function and catalysis are obscure. Simplified synthetic protein scaffolds offer a means to avoid such complexity, learn the underlying principles behind the assembly of function and render the modular assembly of enzymatic function a tangible reality. A key feature of such protein design is the control and exclusion of wat...
متن کاملDetermination of de novo synthesized amino acids in cellular proteins revisited by 13C NMR spectroscopy.
13C nuclear magnetic resonance spectroscopy was used to determine the absolute amounts to de novo synthesized amino acids in both the perchloric acid extracts and the hydrolyzed protein fractions of F98 glioma cells incubated for 2 h with 5 mmol/l [U-13C]glucose. 13C NMR spectra of the hydrolyzed protein fraction revealed a marked incorporation of 13C-labelled alanine, aspartate and glutamate i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Accounts of Chemical Research
سال: 2019
ISSN: 0001-4842,1520-4898
DOI: 10.1021/acs.accounts.9b00032